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recombinant human ace2 protein  (Sino Biological)


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    Structured Review

    Sino Biological recombinant human ace2 protein
    Recombinant Human Ace2 Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+ace2+protein/Human+ACE2+%2F+Angiotensin-Converting+Enzyme+2+Protein/10__1016_slash_j__snb__2026__139821-55-0-39
    Average 96 stars, based on 149 article reviews
    recombinant human ace2 protein - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Recombinant:

    Article Title: NRICM101 ameliorates SARS-CoV-2–S1-induced pulmonary injury in K18-hACE2 mice model
    Article Snippet: .. We then added recombinant human ACE2 protein (0.2 μg/mL; Sino Biological, Beijing, China) to the wells and incubated the plate for 40 min at 37°C. .. Finally, we incubated it with rabbit anti-human IgG-HRP for a further 40 min, and then quantified the intensity of the signal as optical density at 450 nm using a SPECTROstar Nano microplate reader (BMG LABTECH, Ortenberg, Germany).

    Article Title: NRICM101 ameliorates SARS-CoV-2-S1-induced pulmonary injury in K18-hACE2 mice model.
    Article Snippet: .. We then added recombinant human ACE2 protein (0.2 μg/mL; Sino Biological, Beijing, China) to the Frontiers in Pharmacology frontiersin.org03 wells and incubated the plate for 40 min at 37°C. .. Finally, we incubated it with rabbit anti-human IgG-HRP for a further 40 min, and then quantified the intensity of the signal as optical density at 450 nm using a SPECTROstar Nano microplate reader (BMG LABTECH, Ortenberg, Germany).

    Article Title: Electrokinetic focusing of SARS-CoV-2 spike protein via ion concentration polarization in a paper-based lateral flow assay.
    Article Snippet: Naon peruorinated resin solution, 20 wt%, Bis-Tris propane, and Pluronic F-127 were purchased from Sigma-Aldrich (St. Louis, MO). .. Recombinant Human ACE2 protein with an mFc Tag (Cat# 10108-H05H) and SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein (Cat# 40591- V08H) were purchased from Sino Biological (Beijing, China). .. Tween-20, Whatman lter paper Grade 1, and Tris$HCl (1.0 M solution), were purchased from Fisher Scientic (Waltham, MA).

    Article Title: Binding of human ACE2 and RBD of Omicron enhanced by unique interaction patterns among SARS-CoV-2 variants of concern.
    Article Snippet: .. Recombinant human ACE2 protein (GenBank accession: AF291820.1, Sino Biological 10108-H08H, Wayne, PA) was labeled with RED-NHS (2nd Generation) dye using the Monolith Protein Labeling Kit (NanoTemper Technologies, MO-L011). .. Labeled ACE2 (5 nM, final concentration) was mixed with the RBD proteins in a serial 15-step and two-fold dilution starting from 4 μM (for WT) or 1 (for Omicron) μM in PBS buffer supplanted with 0.1% Pluronic® F-127.

    Article Title: Multi-phase SERS detection of SARS-CoV-2 from liquids to aerosols and pseudovirus
    Article Snippet: Rapid, ultrasensitive, and specific detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remains challenging, particularly for micro-volume samples.. This study presents a capillary-based threedimensional Ag/ZnO nanorod surface-enhanced Raman spectroscopy (SERS) biosensing platform that enables dual-mode (liquid and aerosol) viral detection within the same device.. The capillary configuration inherently provides a large surface-to-volume ratio and acts as a confined reaction chamber, eliminating the need for external liquid or gas cells while minimizing sample consumption.

    Article Title: Characterization of private mutations in the spike protein of SARS-CoV-2 correlates with viral prevalence
    Article Snippet: The receptor protein Recombinant Human ACE2 Protein (His & AVI Tag), Biotinylated (Sino Biological, Beijing, China) was immobilized at a concentration of 25nM on streptavidin biosensor (SA) (Sartorius, Germany). .. Recombinant Human ACE2 Protein (His & AVI Tag), Biotinylated (Sino Biological, Beijing, China), and SARS-CoV-2 EG.5.1 spike-L455F (synthesized by Sino Biological, Beijing, China) were tested for molecular interactions at concentrations of 500nM, 250nM, 125nM, 62.5nM and 31.25nM, respectively. .. The biomolecular interaction system OctetRED96 (ForteBio, America) was used to measure interaction signals for SARS-CoV-2 spike protein, with three independent replicates performed for each concentration.

    Article Title: Development of Receptor-Integrated Magnetically Labeled Liposomes for Investigating SARS-CoV-2 Fusion Interactions
    Article Snippet: Croda International provided 1,2-Dioleoyl-sn-glycero-3phosphocholine (DOPC) and ovine cholesterol (ovine wool, >98%), and recombinant SARS-CoV-2 spike protein (ab69741) was obtained from Abcam. .. Anti-SARS-CoV-2 monoclonal antibody and recombinant human ACE2 protein with an His-tag were obtained from SinoBiological. .. Polyacrylic acid (PAA) was obtained from Sigma-Aldrich.

    Incubation:

    Article Title: NRICM101 ameliorates SARS-CoV-2–S1-induced pulmonary injury in K18-hACE2 mice model
    Article Snippet: .. We then added recombinant human ACE2 protein (0.2 μg/mL; Sino Biological, Beijing, China) to the wells and incubated the plate for 40 min at 37°C. .. Finally, we incubated it with rabbit anti-human IgG-HRP for a further 40 min, and then quantified the intensity of the signal as optical density at 450 nm using a SPECTROstar Nano microplate reader (BMG LABTECH, Ortenberg, Germany).

    Article Title: NRICM101 ameliorates SARS-CoV-2-S1-induced pulmonary injury in K18-hACE2 mice model.
    Article Snippet: .. We then added recombinant human ACE2 protein (0.2 μg/mL; Sino Biological, Beijing, China) to the Frontiers in Pharmacology frontiersin.org03 wells and incubated the plate for 40 min at 37°C. .. Finally, we incubated it with rabbit anti-human IgG-HRP for a further 40 min, and then quantified the intensity of the signal as optical density at 450 nm using a SPECTROstar Nano microplate reader (BMG LABTECH, Ortenberg, Germany).

    Labeling:

    Article Title: Binding of human ACE2 and RBD of Omicron enhanced by unique interaction patterns among SARS-CoV-2 variants of concern.
    Article Snippet: .. Recombinant human ACE2 protein (GenBank accession: AF291820.1, Sino Biological 10108-H08H, Wayne, PA) was labeled with RED-NHS (2nd Generation) dye using the Monolith Protein Labeling Kit (NanoTemper Technologies, MO-L011). .. Labeled ACE2 (5 nM, final concentration) was mixed with the RBD proteins in a serial 15-step and two-fold dilution starting from 4 μM (for WT) or 1 (for Omicron) μM in PBS buffer supplanted with 0.1% Pluronic® F-127.

    Purification:

    Article Title: Multi-phase SERS detection of SARS-CoV-2 from liquids to aerosols and pseudovirus
    Article Snippet: Rapid, ultrasensitive, and specific detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remains challenging, particularly for micro-volume samples.. This study presents a capillary-based threedimensional Ag/ZnO nanorod surface-enhanced Raman spectroscopy (SERS) biosensing platform that enables dual-mode (liquid and aerosol) viral detection within the same device.. The capillary configuration inherently provides a large surface-to-volume ratio and acts as a confined reaction chamber, eliminating the need for external liquid or gas cells while minimizing sample consumption.

    Synthesized:

    Article Title: Characterization of private mutations in the spike protein of SARS-CoV-2 correlates with viral prevalence
    Article Snippet: The receptor protein Recombinant Human ACE2 Protein (His & AVI Tag), Biotinylated (Sino Biological, Beijing, China) was immobilized at a concentration of 25nM on streptavidin biosensor (SA) (Sartorius, Germany). .. Recombinant Human ACE2 Protein (His & AVI Tag), Biotinylated (Sino Biological, Beijing, China), and SARS-CoV-2 EG.5.1 spike-L455F (synthesized by Sino Biological, Beijing, China) were tested for molecular interactions at concentrations of 500nM, 250nM, 125nM, 62.5nM and 31.25nM, respectively. .. The biomolecular interaction system OctetRED96 (ForteBio, America) was used to measure interaction signals for SARS-CoV-2 spike protein, with three independent replicates performed for each concentration.



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    R&D Systems ace2 protein
    ( A ) Quantification of <t>ACE2,</t> on lungs, by ELISA in uninfected mice treated or not with Losartan or Losartan + Lisinopril combination over 21 days (black and white columns; UU: uninfected untreated; CTU: combination-treated, uninfected; LTU: Losartan-treated, uninfected) and in the same animals after infection with SARS-CoV-2 (colored columns; CTI [gray]: combination-treated, infected; LTI [green]: Losartan-treated, infected; UI [red]: untreated, infected). ( B ) Viral load in SARS-CoV-2-infected K18-hACE2 mice treated or not with Losartan or Losartan-Lisinopril combination on days 3, 5, or 6/7 post-infection. (*) Significant difference between groups: * p < 0.05, ** p < 0.005, **** p < 0.0001. The normality of the data was assessed using the Shapiro–Wilk test. A one-way ANOVA was performed, followed by the Bonferroni post-test (A-Treated non-infected), and mixed-effects analyses were conducted, followed by the Tukey post-test (A-Treated infected/B). (*) Significant difference between specific groups as indicated by the lines: * p < 0.05, ** p < 0.005, **** p < 0.0001. Data are presented as mean ± SEM. n = 10 per time point.
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    Image Search Results


    ( A ) Quantification of ACE2, on lungs, by ELISA in uninfected mice treated or not with Losartan or Losartan + Lisinopril combination over 21 days (black and white columns; UU: uninfected untreated; CTU: combination-treated, uninfected; LTU: Losartan-treated, uninfected) and in the same animals after infection with SARS-CoV-2 (colored columns; CTI [gray]: combination-treated, infected; LTI [green]: Losartan-treated, infected; UI [red]: untreated, infected). ( B ) Viral load in SARS-CoV-2-infected K18-hACE2 mice treated or not with Losartan or Losartan-Lisinopril combination on days 3, 5, or 6/7 post-infection. (*) Significant difference between groups: * p < 0.05, ** p < 0.005, **** p < 0.0001. The normality of the data was assessed using the Shapiro–Wilk test. A one-way ANOVA was performed, followed by the Bonferroni post-test (A-Treated non-infected), and mixed-effects analyses were conducted, followed by the Tukey post-test (A-Treated infected/B). (*) Significant difference between specific groups as indicated by the lines: * p < 0.05, ** p < 0.005, **** p < 0.0001. Data are presented as mean ± SEM. n = 10 per time point.

    Journal: International Journal of Molecular Sciences

    Article Title: Angiotensin-Converting Enzyme Inhibition and/or Angiotensin Receptor Blockade Modulate Cytokine Profiles and Improve Clinical Outcomes in Experimental COVID-19 Infection

    doi: 10.3390/ijms26167663

    Figure Lengend Snippet: ( A ) Quantification of ACE2, on lungs, by ELISA in uninfected mice treated or not with Losartan or Losartan + Lisinopril combination over 21 days (black and white columns; UU: uninfected untreated; CTU: combination-treated, uninfected; LTU: Losartan-treated, uninfected) and in the same animals after infection with SARS-CoV-2 (colored columns; CTI [gray]: combination-treated, infected; LTI [green]: Losartan-treated, infected; UI [red]: untreated, infected). ( B ) Viral load in SARS-CoV-2-infected K18-hACE2 mice treated or not with Losartan or Losartan-Lisinopril combination on days 3, 5, or 6/7 post-infection. (*) Significant difference between groups: * p < 0.05, ** p < 0.005, **** p < 0.0001. The normality of the data was assessed using the Shapiro–Wilk test. A one-way ANOVA was performed, followed by the Bonferroni post-test (A-Treated non-infected), and mixed-effects analyses were conducted, followed by the Tukey post-test (A-Treated infected/B). (*) Significant difference between specific groups as indicated by the lines: * p < 0.05, ** p < 0.005, **** p < 0.0001. Data are presented as mean ± SEM. n = 10 per time point.

    Article Snippet: Detection and quantification of ACE2 protein in tissue samples were performed using the Human ACE2 DuoSet ELISA kit (R&D Systems- Cat. DY933-05, Minnneapolis, MN, USA), following the manufacturer’s recommendations.

    Techniques: Enzyme-linked Immunosorbent Assay, Infection